Tag Archives: sarcoidosis

Antiphospholipid Antibodies: Discussion

Antiphospholipid Antibodies: DiscussionTherefore, the presence of immune type aPL is thought to be responsible for the occurrence of thrombotic complications because foGPI is a well-known plasma protein with anticoagulant activity.
The enzyme-linked immunosorbent assay method we applied in the present study for the detection of aPL can detect these two types of aPL, but cannot distinguish them. Therefore, it is unknown whether aPL detected in patients with sarcoidosis in the present study belongs to the autoimmune type or the infective type other asthma inhalers online. In sarcoidosis, arterial or venous thrombosis and recurrent spontaneous fetal loss do not usually occur, suggesting that the aPL detected in sarcoidosis is mainly the infective type.
In tuberculosis, another epithelioid cell granuloma-forming disease, the presence of infective type aPL also has been reported. The fact that the cell membrane of patients who have Mycobacterium tuberculosis-caused tuberculosis is mainly composed of phospholipids is thought to be responsible for the induction of aPL in tuberculosis. The same mechanism in syphilis is considered responsible for the induction of aPL. Therefore, bacterial infection may well be involved in the induction of aPL in sarcoidosis. However, further study will be necessary to clarify this.
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Antiphospholipid Antibodies: Analysis

There were 23 cases in which abnormal chest x-ray film findings disappeared within 2 years, but 23 cases in which these findings persisted for over 2 years. The aPL frequency was 13 percent (3 of 23) in the former group and 65 percent (15 of 23) in the latter. Nine cases not included in this analysis were either cases in which observations were made for fewer than 2 years or cases which were diagnosed as stage 0 on the first visit. The data demonstrate a significantly higher aPL frequency in the group with abnormal findings for more than 2 years (p<0.01), as seen in Table 2.
Thirty-one cases were observed, this time for more than 5 years. In 16 of them, abnormal findings disappeared within 5 years, but persisted in the other 15 for more than 5 years. The frequency of aPL was 19 percent (3 of 16) in the former group and 73 percent (11 of 15) in the latter. http://birthcontroltab.com other Thus, aPL occurred significantly more often in the group with abnormal findings for more than 5 years (p<0.01), as seen in Table 2.
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Antiphospholipid Antibodies: Results

Antiphospholipid Antibodies: ResultsFrequency of Antiphospholipid Antibodies
Twenty-one (9 male and 12 female) of 55 (38 percent) cases of sarcoidosis had aPL (IgG or IgM aPL or both). In contrast, only 5 (7 percent) of 70 cases in the control group were positive for aPL. The presence of aPL was significantly higher (p<0.01) in patients with sarcoidosis (Fig 1).
Regarding the classes of aPL, 10 cases (18 percent), 4 male and 6 female, had IgG aPL and 16 (29 percent), 7 male and 9 female, had IgM aPL. No significant difference was found between the frequency of IgG or IgM aPL in males (4 of 18 [32 percent] or 7 of 18 [39 percent], respectively) and that in females (6 of 37 [16 percent] or 9 of 37 [24 percent], respectively), as seen in Table 1. No particular antibody in either IgG or IgM was detected with significantly high incidence (Fig 1).
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Antiphospholipid Antibodies: Methods

The IgG and IgM antibodies against each phospholipid were measured by an enzyme-linked immunosorbent assay method. Microtiter plates were coated with each type of phospholipid and diluted with methanol to 50 Mg/ml. After drying at 50°C for 30 min and washing 3 times in 0.01 mol/L phosphate-buffered saline (PBS), 50 Mg/ml of 10 percent bovine serum was added to each well and kept at room temperature for 1 h in order to eliminate adhesion nonspecific IgG and IgM. Then 50 ц\ of sera diluted to a 1:100 solution with PBS was placed in each well and incubated at room temperature for 1 h and washed 3 times in PBS. Next, 50 д1 each of peroxidase-labeled antihuman IgG antibodies and IgM antibodies was diluted to a 1:100 solution with PBS. Fifty microliters of Нг02-0-рЬепу1епе diamine was added as a substrate to the wells and incubated at room temperature for 15 min, followed by the addition of 50 д1 of IN H2SO4 to each well to stop the reaction.

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Antiphospholipid Antibodies: Materials

Antiphospholipid Antibodies: MaterialsSarcoidosis is a systemic disease of unknown cause characterized by the presence of non-caseating epithelioid cell granulomas in affected organs and shows a variety of clinical courses. It is well-known that patients with sarcoidosis have humoral immunologic abnormalities. Canadian helth& care mall Increases in serum immunoglobulin and complications of autoimmune diseases also have been reported. Some autoantibodies including antinuclear antibody, rheumatoid factor, and antilymphocyte antibody, also have been detected. However, the clinical significance of these antibodies now is the subject of considerable discussion.
Recently, serum antibodies against phospholipids (antiphospholipid antibodies, [aPL]) comprising cell membrane have been reported to be present in some autoimmune diseases and infectious diseases, and the presence of aPL has been shown to be associated with thromboembolic manifestations such as pulmonary embolism, cerebral infarction, and neurologic defects.
The purpose of this study is to detect aPL and to elucidate their relationship, if any, to the clinical manifestation of patients with sarcoidosis.
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HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: Conclusion

HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: ConclusionWith regard to the association of HLA-radiologic stage of sarcoidosis, the most important deviations have been found in the group of patients in stage I (Table 3). HLA-B8,DR3 shows a higher frequency in stage I with respect to healthy control subjects and patients with stage III sarcoid. We are reminded that HLA-B8 seems to be associated with a good prognosis, and it could not be a simple coincidence if people possessing this allele belong to the radiologic stage I, in which a good outcome is more frequent with respect to other radiologic stages.
Concerning sex of patients and extent of the disease, we noticed several interesting associations, but none of these reached the significance when the p value was corrected for the number of antigens tested. cialis professional 20 mg

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HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: Discussion

Though available data are too scant to draw a worldwide map of HLA-sarcoidosis associations, some results of our investigation seem to be in agreement with literature findings. We confirm suggestions of European investigators about MHC association witH sarcoidosis: HLA-B8 appears to be positively related to the disease in whites (Table 2). These data could not be confirmed in West Indian or Japanese patients, the latter belonging to a population where B8 is a very rare antigen. It is known that HLA-B8,DR3 antigens may be linked to disorders with an immunologic basis and that HLA-B8,DR3 positive individuals may undergo the development of diseases with an autoimmune component. Both these observations fit the current concepts on the pathogenesis of sarcoidosis. We cannot draw any conclusion in our series about a possible association of HLAr prognosis, as others have previously published, since at the moment of the preparation of this manuscript, most of our patients have not reached a sufficient follow-up period. canadianfamilypharmacy

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HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: Results

HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: ResultsCharacteristics of Patients with Sarcoid
Data are described in Table 1. In our series, the female:male ratio was 1.48:1. The sample study enrolled a large majority of nonsmokers (69.15 percent). Only 37 patients (34.57 percent) were asymptomatic (chest radiographic abnormalities noticed during periodic screening), whereas most of patients (65.42 percent) had symptoms (dyspnea, fever, erythema nodosum, arthralgia, cough, chest pain, or ophthalmic symptoms). Radiologic stage II assembled 40 patients (37.38 percent), whereas 38 patients (35.51 percent) were assigned to stage I, and 25 (23.36 percent) were assigned to stage III. Finally, 38 patients (35.51 percent) had one or more extrapulmonary localizations of the disease.
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HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis: Methods

Subjects
We studied 107 patients (64 female and 43 male) with newly recognized, biopsy specimen-proven sarcoidosis admitted to our departments (Pavia and Padova) from May 1988 to April 1991. The mean age at the onset of the disease was 36.08 years (39.3 years for female subjects and 31.13 years for male subjects), ranging from 18 to 64 years. As controls, the HLA phenotype frequencies obtained in 510 healthy individuals from continental Italy and reported in the Data Book Addendum—Anthropological Analysis, from the 11th Histocompatibility Workshop book were employed. BF allotyping was performed in 382, and C4A and C4B allotyping in 370 healthy northern Italian control subjects.
Assessment of Sarcoidosis
Patients with sarcoid were assessed by bronchoalveolar lavage, Ga lung scanning with computer-assisted score expressed Ga uptake, pulmonary function tests, and serum angiotensin-converting enzyme activity. The chest radiograph was evaluated according to De Remee and patients were assigned to stage I (bilateral hilar lymphoadenopathy [BHL], II (BHL and diffuse pulmonary involvement), or III (diffuse pulmonary involvement only). Typing for HLA class I and II antigens was performed in all patients with a set of sera, whose reliability was proven during the Tenth International Histocompatibility Workshop, using the National Institutes of Health microlymphocytotoxicity method. The technique of resetting with sheep erythrocytes (SRBC) was employed to remove T cells and obtain enriched В-cell suspensions, as previously described.
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HLA Class I, II, and III Polymorphism in Italian Patients With Sarcoidosis

HLA Class I, II, and III Polymorphism in Italian Patients With SarcoidosisSarcoidosis is a multisystem granulomatous disorder characterized by an accumulation of immunocompetent cells at sites of disease activity. Though the etiology is unknown, both prevalence of sarcoidosis in different ethnic groups and familial occurrence of the disease strongly put forward the hypothesis of a possible genetic predisposition.
Frequencies of human leukocyte antigens (HLA) coded by genes of the HLA region in patients with sarcoidosis have been studied by many investigators in order to understand the role of immunogenetic factors in the pathogenesis of this disease. Despite the relatively large body of published articles, a final conclusion on the association of sarcoidosis and HLA has not been reached. Conflicting results were achieved when different ethnic groups were considered, so that, for instance, from pooled data an association between HLA and sarcoidosis in Caucasians has been denied. When another ethnic group or narrower caucasian groups were considered, the existence of association between sarcoidosis and certain HLA alleles, especially B8 in Caucasians, has been recognized. This implies the presence of a putative sarcoidosis-susceptibility gene within or linked to the HLA region.
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