Category Archives: Antiphospholipid Antibodies

Antiphospholipid Antibodies: Discussion

Antiphospholipid Antibodies: DiscussionTherefore, the presence of immune type aPL is thought to be responsible for the occurrence of thrombotic complications because foGPI is a well-known plasma protein with anticoagulant activity.
The enzyme-linked immunosorbent assay method we applied in the present study for the detection of aPL can detect these two types of aPL, but cannot distinguish them. Therefore, it is unknown whether aPL detected in patients with sarcoidosis in the present study belongs to the autoimmune type or the infective type other asthma inhalers online. In sarcoidosis, arterial or venous thrombosis and recurrent spontaneous fetal loss do not usually occur, suggesting that the aPL detected in sarcoidosis is mainly the infective type.
In tuberculosis, another epithelioid cell granuloma-forming disease, the presence of infective type aPL also has been reported. The fact that the cell membrane of patients who have Mycobacterium tuberculosis-caused tuberculosis is mainly composed of phospholipids is thought to be responsible for the induction of aPL in tuberculosis. The same mechanism in syphilis is considered responsible for the induction of aPL. Therefore, bacterial infection may well be involved in the induction of aPL in sarcoidosis. However, further study will be necessary to clarify this.
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Antiphospholipid Antibodies: Analysis

There were 23 cases in which abnormal chest x-ray film findings disappeared within 2 years, but 23 cases in which these findings persisted for over 2 years. The aPL frequency was 13 percent (3 of 23) in the former group and 65 percent (15 of 23) in the latter. Nine cases not included in this analysis were either cases in which observations were made for fewer than 2 years or cases which were diagnosed as stage 0 on the first visit. The data demonstrate a significantly higher aPL frequency in the group with abnormal findings for more than 2 years (p<0.01), as seen in Table 2.
Thirty-one cases were observed, this time for more than 5 years. In 16 of them, abnormal findings disappeared within 5 years, but persisted in the other 15 for more than 5 years. The frequency of aPL was 19 percent (3 of 16) in the former group and 73 percent (11 of 15) in the latter. other Thus, aPL occurred significantly more often in the group with abnormal findings for more than 5 years (p<0.01), as seen in Table 2.
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Antiphospholipid Antibodies: Results

Antiphospholipid Antibodies: ResultsFrequency of Antiphospholipid Antibodies
Twenty-one (9 male and 12 female) of 55 (38 percent) cases of sarcoidosis had aPL (IgG or IgM aPL or both). In contrast, only 5 (7 percent) of 70 cases in the control group were positive for aPL. The presence of aPL was significantly higher (p<0.01) in patients with sarcoidosis (Fig 1).
Regarding the classes of aPL, 10 cases (18 percent), 4 male and 6 female, had IgG aPL and 16 (29 percent), 7 male and 9 female, had IgM aPL. No significant difference was found between the frequency of IgG or IgM aPL in males (4 of 18 [32 percent] or 7 of 18 [39 percent], respectively) and that in females (6 of 37 [16 percent] or 9 of 37 [24 percent], respectively), as seen in Table 1. No particular antibody in either IgG or IgM was detected with significantly high incidence (Fig 1).
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Antiphospholipid Antibodies: Methods

The IgG and IgM antibodies against each phospholipid were measured by an enzyme-linked immunosorbent assay method. Microtiter plates were coated with each type of phospholipid and diluted with methanol to 50 Mg/ml. After drying at 50°C for 30 min and washing 3 times in 0.01 mol/L phosphate-buffered saline (PBS), 50 Mg/ml of 10 percent bovine serum was added to each well and kept at room temperature for 1 h in order to eliminate adhesion nonspecific IgG and IgM. Then 50 ц\ of sera diluted to a 1:100 solution with PBS was placed in each well and incubated at room temperature for 1 h and washed 3 times in PBS. Next, 50 д1 each of peroxidase-labeled antihuman IgG antibodies and IgM antibodies was diluted to a 1:100 solution with PBS. Fifty microliters of Нг02-0-рЬепу1епе diamine was added as a substrate to the wells and incubated at room temperature for 15 min, followed by the addition of 50 д1 of IN H2SO4 to each well to stop the reaction.

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Antiphospholipid Antibodies: Materials

Antiphospholipid Antibodies: MaterialsSarcoidosis is a systemic disease of unknown cause characterized by the presence of non-caseating epithelioid cell granulomas in affected organs and shows a variety of clinical courses. It is well-known that patients with sarcoidosis have humoral immunologic abnormalities. Canadian helth& care mall Increases in serum immunoglobulin and complications of autoimmune diseases also have been reported. Some autoantibodies including antinuclear antibody, rheumatoid factor, and antilymphocyte antibody, also have been detected. However, the clinical significance of these antibodies now is the subject of considerable discussion.
Recently, serum antibodies against phospholipids (antiphospholipid antibodies, [aPL]) comprising cell membrane have been reported to be present in some autoimmune diseases and infectious diseases, and the presence of aPL has been shown to be associated with thromboembolic manifestations such as pulmonary embolism, cerebral infarction, and neurologic defects.
The purpose of this study is to detect aPL and to elucidate their relationship, if any, to the clinical manifestation of patients with sarcoidosis.
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